Search results for "Tagged primer method"

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Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing.

2015

Eight microsatellite loci were characterized for Meoma ventricosa (Lamarck, 1816), a burrowing sea urchin that can be afflicted by a bacterial disease causing localized mass mortality. For the analyzed population (29 individuals from St. Croix, US Virgin Islands), we observed 8.125 mean number of alleles, 0.640 mean observed heterozygosity (Ho) and 0.747 mean expected heterozygosity (He). Two loci showed significant deviations from Hardy-Weinberg equilibrium. Overall, the described loci were characterized by a moderately highlevel of polymorphism suggesting that these markers are useful for a population genetic studyin the Caribbean Sea.

0106 biological sciencesPopulationZoology454 method010603 evolutionary biology01 natural sciencesBiochemistryLoss of heterozygosityMeoma ventricosa03 medical and health sciencesbiology.animal14. Life underwaterAlleleeducationMicrosatellitesSea urchinTagged primer methodEcology Evolution Behavior and Systematics030304 developmental biologySpatangoidaGeneticsCaribbean0303 health scienceseducation.field_of_studyBacterial diseasebiology[ SDV.GEN.GA ] Life Sciences [q-bio]/Genetics/Animal geneticsbiology.organism_classification[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal geneticsMicrosatelliteEchinoidSciences exactes et naturelles

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